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15 To further verify whether the increase in cholesterol levels induced by cigarette smoke in airway epithelial cells contributes to cigarette smoke-induced airway inflammation through ROS promotion, we treated HBE cells with water-soluble cholesterol and detected ROS levels in airway epithelial cells using flow cytometry (Figure 6A) and the secretion levels of inflammatory factors IL6 and IL8 using qPCR (Figure 6B)

The pens also tended to contain more nicotine

Make sure to open all the windows, and leave them open for several hours to properly air it out

6. Estimated IR Voltage Drop