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Real-time PCR Total RNA was isolated from the cells using the Roche High Pure RNA isolation kit (Roche Life Science, 11828665001) and reverse transcription was performed using the iScript cDNA synthesis kit (Bio-Rad, 1708891) according to the manufacturers instructions

Supports the reduction of systemic and localized inflammation without suppressing immune function

PARP inhibitors: synthetic lethality in the clinic

Evidence from animal models points toward support of axonal continuity, synaptic stability, and functional recovery following nervous system injury

Metformin is widely used off-label to reduce insulin resistance, lower fasting insulin levels, improve metabolic flexibility, and activate longevity pathwayseven in non-diabetic individuals