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The intranasal (nose-to-brain) administration method offers a number of advantages, as it avoids first pass effect, is less intrusive, provides a broad surface area for absorption, and reduces nontargeted delivery to minimize systemic exposure [7, 25]

For the luciferase assays, 5 L of the supernatant was diluted with 95 L PBS (pH 7.4) in white flat-opaque-bottomed 96-well plates (Thermo Fisher Scientific)

Filtration removes some contaminants but doesn't sterilize

Endocrine Metab Immune Disord Drug Targets

Reduced glutathione reflects both antioxidant capacity and the functional performance of key metabolic pathways involved in sulfur metabolism and methylation balance