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For protection assays, crude venom was preincubated (30 min at 37 C) with varying cytotoxin:binder ratios of 1:1, 1:2.5 and 1:5 before injection ( n = 3) (ratios estimated from the proportion of cytotoxin in the venom)
The Hollywood A-lister who got smashed on cheap cigarettes watching Elton John, Rod Stewart and Nazareth in 1974 Gigs today ain't what they used to be In a reminder of the way things used to be, Hollywood A-lister Hugh Grant has reminisced about an early concert experience that couldn't be repeated today
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Ethics approval The study is registered at ClinicalTrials.gov (NCT03822546) and a favourable ethical opinion of this study was given by the Institutional Review Board of Chesapeake Research Review (Maryland, USA)
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