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The cells were grown in Endothelial Cell Medium (ECM, ScienCell Research Laboratories, Carlsbad, CA, USA) supplemented with 10% FBS, 1% (v/v) penicillin/streptomycin, and 1% endothelial cell growth factors at 37 C with 5% CO 2 and 95% air

In women, smoking has been linked with: increased difficulty getting pregnant early birth, stillbirth low birthweight sudden infant death syndrome (SIDS) ectopic pregnancy cleft lips In addition to all the health effects listed above, smoking cigarettes can make bones weaker, cause tooth loss and other gum and tooth diseases, increase a persons risk of developing cataracts and macular degeneration, causes type 2 diabetes, makes the immune system weaker, and can cause arthritis

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