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After pretreatment, sections were incubated with 5% skimmed milk for 15 min, followed by 1-h incubation with primary antibodies

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Randomised clinical trial: efficacy of Lactobacillus paracasei-enriched artichokes in the treatment of patients with functional constipation--a double-blind, controlled, crossover study

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Follow your labs SOPs for storage conditions and stability handling