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In redox signalling research, GSH participates in protein glutathionylation, a reversible post-translational modification that protects protein thiol groups under oxidative conditions

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For monitoring cellular GSH levels, all MSCs were loaded with 2 M FreSHtracer for 2 h in the culture medium and subsequently sorted at 4 C according to their F 510 /F 580 levels using an AriaIII Flow Cytometer System (BD Biosciences, San Jose, CA) [8]

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