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Cells resuspended in the growth medium (GM, DMEM containing 10% fetal bovine serum (FBS, Life Technologies) and 1% anti-anti), and the single cells were seeded in a cell culture flask

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Hippocampi from P4 Sprague Dawfey rats were cultured as previously described (Wayman, Impey et al

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How Alcohol-Related Nutrient Deficiencies Drive Brain, Immune, Gut, and Liver Dysfunction Nutrient deficiencies caused by alcohol do not remain isolated biochemical problems