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From this preliminary screening, we selected 43 compounds that showed at least 50% inhibition in all three assays for further doseresponse studies (Supplementary Tables 26)

Zhu et al

Transient Transfection of Human GSTP1 Gene After plating on collagen-coated plates for 24 h, HepG2 cells were transiently transfected with 0.1 g/1 10 4 cells hGSTP1 expression plasmid (SC119655, Origene, Rockville, MD, United States) or accompanying empty pCMV6-XL5 vector (pCMV6-XL5) using the GenJet In Vitro Transfection Reagent for HepG2 cells (SignaGen, Rockville, MD, United States) according to the manufacturers instructions

Increase your intake of methylation nutrients The methylation nutrients include vitamins B6, B9, B12, and folate

Glutathione also supports healthy immune function and plays an important role in maintaining cellular health throughout the body