Chou, C
FOXO4-DRI (also known as Proxofim) is a synthetic peptide with a retro-inverso structure, developed by Dutch researchers as a modified version of the FOXO4 protein with an extended half-life
references cited by the guest are included where identifiable
Section 5 says
(14) (14) Preparation Fmoc-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin Fmoc-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (13)Fmoc-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Glu(otBu)-OH1.1gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml1.53DMF3DMF2Fmoc-Glu(otBu)- Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin In the Fmoc-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin of step (13), add decapping reagent ( 20% piperidine in DMF), reacted at room temperature for 5 minutes, drained, re-added capping agent and reacted at room temperature for 20 minutes, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with distilled DMF, drained , add Fmoc-Glu(otBu)-OH1.1g, DIEA0.4ml, TBTU0.56g, HOBT0.2g, DMF10ml, react at room temperature for 1.5 hours, dry with a vacuum pump, wash with isopropanol for 3 times, DMF for 3 times, repeat The steamed DMF was washed twice and dried to obtain Fmoc-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly- Leu-Val-Wang Resin
