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For a 5 mg target dose: Volume to Draw = Desired DoseConcentration = 5 mg10 mg/mL = 0.5 mL Convert to syringe units

Prior to sample loading the C18 disk was prepared by sequential treatment with methanol (20 L), acetonitrile/water/TFA (solvent B, 20 L, 80/20/0.1, v/v/v) and finally with solvent A (20 L)

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