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The peptide exists physiologically as a complex with copper(II) ions and is found in saliva, urine, and plasma, where its concentration declines significantly with age

the culture medium was exchanged by an extracellular solution containing (in mM) 140 NaCl, 2.5 KCl, 1 MgCl 2 , 3 CaCl 2 , 25 glucose, and 5 HEPES

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