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These limitations may be better addressed through prospective studies enriched with real-time physiological data, which could further validate the findings of the present study

The synergy activates MAPK signaling pathways To determine if the activation of MAPK signaling pathways is involved in the synergy between CSE and SSL, the levels of total and phosphorylated p38 MAPK, ERK1/2 and JNK were analyzed by western blot on proteins extracted from the epidermis of the skin substitutes (Fig