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Hydrophobic BAs, such as chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA), have been shown to impair epithelial barrier function by disrupting the expression and structural integrity of tight junction proteins, including Occludin, ZO-1, and Claudins 151

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Method selection should be driven by the required conjugation site, substrate electrophilicity, oxidation sensitivity, and whether the customer needs a metabolite-like adduct, a preserved free-thiol derivative, or a labeled construct for downstream assay use