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Cells were then incubated with surface antibodies specific for CD45, CD11b, CD11c, and Ly6C antibodies for 30 min at 4 C
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1 H NMR (400 MHz, Chloroform-d) = 8.65 (dd, J = 4.8, 2.6 Hz, 1H), 8.047.93 (m, 2H), 7.83 (td, J = 7.7, 2.2 Hz, 1H), 7.74 (dd, J = 8.1, 2.6 Hz, 1H), 7.32 (p, J = 3.3 Hz, 1H), 7.026.91 (m, 2H), 4.15 (dt, J = 8.6, 3.9 Hz, 2H), 3.83 (dt, J = 8.5, 3.7 Hz, 2H), 2.03 (dt, J = 8.4, 3.9 Hz, 2H)