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Abstract It has remained unknown how cells reduce cystine taken up from the extracellular space, which is a required step for further utilization of cysteine in key processes such as protein or glutathione synthesis

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After three weeks of virus expression, immunofluorescence staining confirmed that EGFP + signal largely co-localized with signal from glutamate-specific antibody in PVN, indicating specific targeting of PVN Glu neurons (Fig