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Using HEK-293 adherent cells and K-562 suspension cells treated with varying concentrations of menadione or l-Buthionine Sulfoximine (BSO), two compounds known to affect total glutathione (GSH + GSSG) levels, we assayed the cells in separate assays and in a multiplex assay

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Example of a glutathione (GSH) difference spectrum from the A) dorsomedial prefrontal cortex (DMPFC) and B) from the medical occipital cortex (mOCC)