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Various methods of administration (oral, topical, and injectable)

Each sample was thereafter incubated with 10 l of acetonitrile and stored at 20 C before analysis was performed on a Beckman HPLC instrument operated with System Gold coupled to diode array UVVIS detector module 168

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Although intracerebroventricular (i.c.v.) administration of GH caused no significant changes in food intake during the first 4 h of measurement, C57BL/6 mice exhibited increased food intake and reduced energy expenditure 24 h after the injection (Fig