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MD simulations demonstrated that, compared to the wild-type PV2 (WT), PV2-C23pAcF exhibited a significantly higher total number of hydrogen bonds in replica 1approximately 80 morewhereas PV2-C96pAcF showed only a slight increase relative to WT (Fig

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This process will consume NADH and compromise the use of NADH for the production of ATP via the electron transport chain