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The 19 restricted peptides spanned nearly every major clinical category in peptide therapy: tissue repair (BPC-157, Thymosin Beta-4), immune modulation (Thymosin Alpha-1, Selank), growth hormone signaling (CJC-1295, Ipamorelin, GHRP-2, GHRP-6), metabolic support (AOD-9604, MOTS-C), cognitive health (Semax, Selank), wound healing (GHK-Cu), and others including KPV, Kisspeptin-10, Melanotan II, PEG-MGF, Epitalon, Cathelicidin LL-37, and Emideltide (DSIP)

Its role in collagen synthesis is particularly vital for maintaining the structural integrity of tissues during recovery from stress

MNC were suspended in 90% fetal calf serum (FCS, Thermo Scientific, Paisley, UK) and 10% dimethyl sulfoxide (DMSO, Origen Biomedical, Solihull, UK) and stored in liquid nitrogen prior to use