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7) Place the plate on an orbital shaker for 1 minute

This intricate nexus, predominantly involving peripherally activated immune cells, notably circulating monocytes, and microglia, along with its repercussions on signaling pathways like serotonin, dopamine, and corticotropin-releasing hormone, with the latters implications on the hypothalamus-pituitaryadrenal axis, initiates sickness behaviors, including fatigue (4, 8)

The ephrins A include six members (A1A6) and are attached to the membrane by a glycosylphosphatidyl-inositol (GPI) domain