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The transcription factor myc controls metabolic reprogramming upon T lymphocyte activation

Experimental approachescell culture A549 cells (human alveolar basal epithelial adenocarcinoma) were maintained at 37 C with 5% CO 2 in RPMI 1640 Medium containing 10% ( v/v ) inactivated fetal calf serum (FCS) and 100 g/mL penicillin-streptomycin

The membranes were blocked with 5% BSA and incubated with primary antibodies at 4 C for 12 h, followed by secondary antibodies (HRP-conjugated Goat Anti-Mouse IgG, Proteintech, SA00001-1, 1:20000) for 1 h at room temperature

Data lake-driven analytics identify nocturnal non-dipping of heart rate as predictor of unfavorable stroke outcome at discharge

doi: 10.3389/fnins.2024.1370533 76 OpertoF