E., & Nair, K
Introduction The technology of semen cryopreservation effectively promotes the application of technologies including genetic resource conservation, zootechnical management, and artificial insemination ( However, the cold shock and alterations in atmospheric oxygen seriously led to biochemical, biophysical, ultrastructural, molecular, and functional alterations ( To ameliorate oxidative stress-related damage, the supplementations of antioxidants, including vitamins, coenzymes, exosomes, liposomes, nanomaterials and trace elements such as selenium (Se), copper, and zinc to semen extender have been widely conducted ( According to a previous study, the co-supplementation of vitamin E effectively ameliorates the low toxicity threshold of Se ( GSH, an endogenous antioxidant synthesized from different amino acids including glycine, cysteine, and glutamate, effectively inhibits oxidative stress-related damage and altered ROS production level ( in vitro development
In this state, it can withstand ambient shipping temperatures without significant degradation
Commercially, theyre more widely available as pills or powder mixes: the broken-down, piecemeal structure produces a small molecular size that allows the peptide to pass easily through the intestinal lining and avoid complete degradation
The EGFP module in the sorting tag can also be replaced by other fluorescent proteins such as YFP, mCherry, mStrawberry, mOrange et al
