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2017 Cell study used 5 mg/kg intraperitoneal injection three times per week in aged mice, with treatment periods of several weeks the three-times-weekly dosing suggests the peptide maintains sufficient functional concentrations for at least 48-72 hours per dose (PMID: 28340339)

Also discard it if the vial was left unrefrigerated after mixing, froze accidentally, or was handled with questionable sterile technique

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Proc Natl Acad Sci USA (2011) 108:163815