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Plasmid pIVEX_GAA_Omega_eYFP-His was prepared by inserting annealed oligonucleotides 1 and 2 (Additional file 2) containing the T7 promoter and the TMV 5 leader sequence (omega) into pIVEX1.3_eYFP-His using the NspI and NcoI sites

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[8] In 2009, the Family Smoking Prevention and Tobacco Control Act was enacted under the Barack Obama administration, once again setting a federal minimum age of 18 and prohibited the FDA from setting a higher minimum purchase age

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Predominant emphysema phenotype in chronic obstructive pulmonary disease patients