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Enzyme-Linked Immunosorbent Assay Analysis Enzyme-Linked Immunosorbent Assay Analysis (ELISA) kits were used to measure the levels of P4HB, CDH13, C1QA, Lactotransferrin (LTF), TNF-, IL-1, cyclic adenosine monophosphate (cAMP), dopamine (DA), 5-hydroxytryptamine (5-HT), and mannan-binding lectin serine protease 2 (MASP2) in serum of patients or mice

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In some embodiments, the otic formulation or composition comprises between about 0.1% to about 40% by weight of the therapeutic agent, or pharmaceutically acceptable prodrug or salt thereof

Unlike direct GH administration, sustained GHS-R1a agonism preserves the pulsatile architecture of GH release a distinction of mechanistic importance in studies of GH secretory dynamics