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The "master antioxidant" simply wasn't making it past the gut

Cell transfection Cells were transfected with pcDNA 3.1 overexpression vectors (pcDNA 3.1-YTHDF1 and pcDNA 3.1-SLC7A5, with the empty pcDNA 3.1 vector as a negative control) and siRNAs (Invitrogen) targeting YTHDF1 and SLC7A5 (si-YTHDF1, si-SLC7A5, with non-targeting siRNA (si-NC) as a negative control)

No drugs were added (A), or 1 mM ATP (B), and in the presence of 1 M A74003 (C) or 5 M BAPTA-AM in avian Muller glia (D)

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