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Methods Cells The 293 T cells (National Collection of Authenticated Cell Cultures, Shanghai, China) were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 100 U/ml penicillin-streptomycin (Gibco) at 37 C with 5% CO 2

doi: 10.1080/02763869.2015.1082382 103

Finally, the relationship between GSH and the immune system was clearly demonstrated in a large study which showed that children with autistic disorder universally have lower natural killer cell activity in peripheral mononuclear cells than those without the disorder which correlated with low intracellular levels of GSH as shown in Table 10[79]

Cells were fixed with 2% paraformaldehyde (15 min), permeabilized with 0.1% Triton X-100 (10 min) and blocked with 2% BSA (1 h)

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