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The mobile phase comprised solvents A (0.1% formic acid in water) and B (0.1% formic acid in 80% acetonitrile), and peptides were fractionated using 555% solvent B at a constant flow rate of 0.30 L/min for 30 min and then loaded onto a Ultimate3000 Nano/Capillary LC System (Thermo Scientific) coupled to a ZenoTOF 7600 mass spectrometer (SCIEX, Framingham, MA, USA)

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Professional support is particularly helpful if there are co-occurring mental health diagnoses, stressful life events happening, or if the person needs support navigating life without weed