The 30 mg vial the low-volume alternative Reconstitute a 30 mg vial with 3 mL of bacteriostatic water: Concentration: 30 mg 3 mL = 10 mg/mL Volume for a 2.5 mg dose: 2.5 mg 10 mg/mL = 0.25 mL Units: 0.25 mL 100 = 25 units The identical 2.5 mg dose that was a full 100-unit barrel from the 5 mg vial is a tidy 25 units from the 30 mg vial a quarter of the volume, and it leaves headroom in the syringe to draw larger doses as the ladder climbs
Store the vial in its original box if possible
For analysis of flavonoids by HPLCMS, reaction volumes were split after the plate reader incubations, and a 40 l aliquot was extracted with 80 l of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid for quantification of cyanidin ( 8 ) concentration while a second 40 L aliquot was extracted with 80 l of 75% acetonitrile in H 2 O, 1 mM EDTA for quantification of all other flavonoids
Briefly, the colon was washed in cold phosphate buffer saline (PBS, 0.01 M, pH 7.4) and homogenized with 10 times of PBS (V/W)
In plain terms, HPLC shows how pure the sample is