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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

However, expression of its receptor PDGFR needs to be tightly controlled, as it is normally downregulated with OL maturation and preventing this via overexpression drives myelin defects 146