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Microelectrode array (MEA) analysis For hCS cultures, 24-well CytoView MEA plates (Axion Biosystems, Atlanta, GA, USA, Cat# M384-tMEA-24W) were prepared 48 h prior to cell plating as follows: wells were first coated with a solution of 0.1% polyethyleneimine (Sigma-Aldrich, Cat# P3143) in borate buffer (pH 8.4) incubated for 1 h at 37 C, rinsed four times with sterile diH 2 O and dried over night at room temperature

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