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Vitamin C also promotes the absorption of iron, while preventing its oxidation

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After two more washes with PBS, biotin was stained with 1 g ml 1 streptavidin-Atto594 (Sigma-Aldrich), and nuclei were stained with NucBlue (Thermo Fisher Scientific, 1:200) in PBS for 60 min at room temperature

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Cut-offs for significance were set to an adjusted p -value of 0.05, and a log2 fold change of 1, with significance subject to BenjaminiHochberg FDR correction