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Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator

It should be noted that eryptotic RBCs are cleared from the circulation in an efferocytosis-dependent fashion within minutes, in contrast to senescent erythrocytes [77], which require days to be engulfed by macrophages and cleared [72]

In the experiment a 4-week prospective and double-blind randomized trial on overweight women found that consuming 25 g of quinoa flakes significantly lowered blood triglycerides, low density lipoprotein and total cholesterol

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