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The carboxyl side chain of glutamic acid is able to act as a donor and acceptor of ammonia, which is toxic to the body, enabling the safe transport of ammonia to the liver where it is converted to urea and excreted by the kidneys

doi: 10.1001/jamanetworkopen.2020.30415

These drawbacks are common to all injections, however, not just vitamin B12

Active at nanomolar concentrations: Unlike many peptides that require high systemic concentrations, GHK-Cu has been shown to exert meaningful biological effects at nanomolar (10 M) concentrations making it effective even at low doses

Regulation of estrogenic effects by beclin 1 in breast cancer cells