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S-glutathionylation of cysteine residues on protein needs to be reversed to maintain homeostasis

Subsequently, the membranes were incubated with the primary antibodies against LDHB (1:10,000, R&D Systems MAB9205-100), LDHA (1:1000, Cell Signaling Technology 2012S), GPX4 (1:1000, Abcam ab125066), DHODH (1: 1000, Cell Signaling Technology 26381S), FSP1 (1:1000, Proteintech 20886-1-AP), SLC7A11 (1:1000, Cell Signaling Technology 12691S), beta-actin (1:5000, Cell Signaling Technology 3700S) overnight on a rotating wheel (3 rpm) at 4 C

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doi: 10.3892/or.2014.3705 81 NasrAYSalehHA

Moreover, research shows that selenium-dependent GPx enzymes, or seleno-GPxs, have synthetic mimics being developed, mainly organoselenium compounds, to replicate their protective effects in therapeutic applications