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If treatment of a cat with oral diazepam is unavoidable, liver enzymes should be checked before and within 5 days after starting therapy

doi: 10.1038/nm.3933 84 FlugeRisaKLundeSAlmeKRekelandIGSapkotaDet al

Immunoblotting The cells were lysed with the Tris lysis buffer containing 10 mM of Tris-HCl, at pH 7.5, 50 mM of NaF, 1% w/v of NP-40, 100 mM of NaCl, 1% sodium deoxycholate, 0.1% w/v SDS, phosphate inhibitors, and protease inhibitors

This study is to explore a GSH-responsive AP probe-net, which can be activated by the abundant GSH in ATC cells to generate reactive alkoxyamine, and the formed alkoxyamine can trap AP sites and block the APE1 activity, leading to ATC cell death but less adverse effects towards normal cells