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Bacterial cells were harvested by centrifugation at 6,000 g for 15 min at 4C and resuspended in lysis buffer (50 mM Tris-HCl, 300 mM NaCl, pH 8.0)

Long-term use is frequently associated with debilitating motor complications, such as dyskinesias and fluctuations in symptom control [6]

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Moreover, the detection rates of several bacterial genera including Collinsella , Lactobacillus and Bacillus were lower than those of non-IBD control group