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Immunofluorescence analysis of phosphorylated Met on cells EHMES-1 cells (2 10 4 cells per well) were seeded in a 96-well glass bottom plate and cultured in RPMI1640 medium supplemented with 10% FBS for 24 h

The optimal timing for oral glutathione is 12 hours before drinking begins (to pre-load hepatic reserves), immediately before bed (to support overnight acetaldehyde clearance), and upon waking (to address residual oxidative stress)

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