For additional stainings, sections were blocked in 3% normal goat serum (Sigma, St Louis, MO) for 1 h, repeatedly washed with PBS and incubated with primary antibodies
Colony Forming Unit (CFU) Count of MRAB Biofilms were treated with either GSH, antibiotics, or a combination of both, for 24 h as described above
Though, several molecular mechanisms amenable for the development of postmenopausal complications has been established, the etiological origin and management of metabolic complications concomitant with these complications remain unclear and the problem needs to be explored ( Figure 2 )
This is an important task in light of the ever-growing prevalence of this disorder while no effective pharmacological treatment has been found so far
doi: 10.1200/JCO.2012.46.1574 77 Savci-HeijinkCDHalfwerkHKosterJvan de VijverMJ