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5 A, B, and Supplementary Fig

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The orientation of NADPH within the crystal structure of the SmTGR (U597C)NADPH complex (Figure 2), along with a previously performed kinetic isotope effect with Pro-S NADPD (Smith et al., 2023), establishes that the hydride transferred to the FAD of SmTGR from NADPH is from the Pro-S position and permits assignment of the observed NMR signals