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In fact, Hans probe could be potentially used as a peroxynitrite probe, similar to the H 2 O 2 probe Hyper, which is reversibly oxidized by H 2 O 2 and reduced by thioredoxin 35

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Sodium chloride 0.9% (80% normal saline) was added to the mixture, briefly vortexed and sonicated one more time using the same conditions as previous

Addition of GSH to the growth media allows these cells to grow by diminishing the levels of ROS, providing a direct link between GSH and protection against ER oxidative stress