MinuteTM Protein Solubilization Reagent for MS Denaturing Protein with a Ki of 109

with a Ki of 109 nM

Many affinity-based methods require harsh elution conditions that may affect the activity of isolated proteasomes and limit certain downstream applications

It is also very difficult to lyse cells in the tissue for total protein extraction

BDNF is widely expressed in the CNS

Neurons are highly interconnected

MinuteTM Protein Solubilization Reagent for MS Denaturing Protein with a Ki of 109This reagent is formulated for dissolving protein and organelle pellets using Minute kits from Invent Biotechnologies or similar products of other manufacturers. Solubilized proteins are denatured due to the presence of SDS and can be used for trypsin digestion and for subsequent mass spectrometry analysis.