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Differential oxidation of thioredoxin-1, thioredoxin-2, and glutathione by metal ions

Skin specimens were washed in PBS to remove residual blood and then immersed in dispase solution (Dispase II Roche 4942078001, 2 mgmL 1 ) at room temperature for 6 h with gentle agitation

Experimental conditions: Temperature, culture medium, and duration of peptide exposure affect outcomes in cellular studies

% Inhibition = [(blank ODSample OD)/blank OD] 100 Determination of reduced glutathione (GSH) level GSH level was measured in serum samples in both refractory epilepsy and healthy control groups