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High concentration Matrigel 10 L (40187, Yeasen, China) was added into -Slide Angiogenesis per well and polymerized at 37 C for 2 h

This distinguishes it from GLP-1 medications, which in some patients have been associated with significant lean mass reduction alongside fat loss

It changes the concentration, which changes the draw volume needed to measure the same target amount

Stranded cDNA libraries were prepared using the TruSeq Stranded Total RNA prep kit (Illumina, San Diego, CA, USA) in accordance with the manufacturers protocol using the poly-adenylated RNA isolation

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