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Subsequently, membranes were incubated overnight at 4 C with the respective primary antibodies diluted in a blocking solution (Table 1)

Considering extensive previous studies reporting that PVN neuronal activity is closely associated with gastrointestinal function 27,28 , we performed immunofluorescence staining to identify the neuronal types in the PVN marked by EGFP: ~ 92% of EGFP + cells were co-stained with a glutamate-specific antibody, while only 12% of EGFP + cells were co-localized with a GABA-specific antibody (Fig

However, in some cases, this approach doesnt fully resolve issues

Exposure to lipid-rich follicular fluid is associated with endoplasmic reticulum stress and impaired oocyte maturation in cumulus-oocyte complexes