For the PVDF membrane, it is completely soaked in methanol for 30 seconds to activate the positively charged groups on the membrane, promoting binding to the negatively charged proteins
Materials and methods CRISPR/Cas9 all-in-one plasmid construction and generation of GLA-KO human iPSCs All-in-one CRISPR/Cas9 carrying GFP and gRNA was purchased from Life Technologies in California, USA (Cat
Herrera-Quintana, L., Vzquez-Lorente, H., Molina-Lpez, J., Gamarra-Morales, Y
Hayakawa, M
Aravinda S, Gopalakrishnan B, Dey CS et al (1995) A testicular protein important for fertility has glutathione S-transferase activity and is localized extracellularly in the seminiferous tubules