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When confluent, HAECs were shifted to ALI culture with 50 L apical media for 8 days to achieve full differentiation with and without IL-13 (10 ng/mL) stimulation (R&D Systems, 213-ILB)

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This phase II clinical trial provided some evidence of benefit on decline in the ALSFRS-R and used a combination of agents to tackle several pathophysiological disease mechanisms

Mechanism of neuroprotective mitochondrial remodeling by PKA/AKAP1