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Studies in animal models demonstrated that TNF knockout mice had higher levels of GC proliferation, less incidence of follicle atresia, and higher fertility than wild-type mice (Cui et al., 2011)

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Cardiac mitochondrial studies Freshly-isolated cardiac mitochondria were used for assessment of mitochondrial function by spectrophotometric assays as previously described 7

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If the accumulation of hydrogen peroxide is not limited, it can diffuse to the cytosol or participate in a chain of reactions that generate more reactive free radicals, such as hydroxyl radical, which can oxidize mitochondrial components (proteins, lipids, and DNA)