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For even more accurate quantification, analyte concentrations in treated cells could be ideally normalized to DNA content or a protein marker reflecting cell viability, given the impact of toxins on cell division over extended periods (24 and 48 h)

Discussions and perspectives Limitations of current evidence, MASLD/ALD differences, and need for clinical translation This review summarizes the role of NAD + in regulating the accumulation of TG, oxidative stress, inflammation, ERS, and fibrosis in cases of fatty liver disease